Abstract:Objective: To observe the analgesic effect of electroacupuncture (EA) on bone cancer pain (BCP) model mice, and to study the effect of electroacupuncture on chemotactic receptor factor 4 (CXCR4) and its downstream inflammatory vesicle signaling pathway 3 (NLRP3) in the spinal cord of BCP mice, and to explore the basis of electroacupuncture to relieve bone cancer pain. mechanism. Methods: C57BL/6 mice were randomly divided into control (control), model (BCP), model + electroacupuncture (BCP+EA), model + electroacupuncture + solvent (BCP+EA+Veh), and model + electroacupuncture + agonist (BCP+EA+SDF-1) groups, with 6 mice in each group. Animal models of femoral cancer pain in C57BL/6 mice were established using Lewis lung cancer cells. Groups requiring electroacupuncture treatment received electroacupuncture at the Sanli and Kunlun points on the left foot on day 8 after modeling, once daily until day 14. Mechanical withdrawal threshold (MWT) for mechanical stimulation and paw withdrawal latency (PWL) for thermal pain stimulation were observed in the left hind paw of mice in each group. The expression of CXCR4, NLRP3, casepase-1, and IL-1β in the spinal cord of mice L4-6 was detected by immunoblotting. Results: Compared with the control group, mice in the BCP group showed a significant decrease in MWT and PWL and a significant increase in CXCR4, NLRP3, casepase-1, and IL-1β expression from day 7; compared with BCP group, BCP+EA group showed significant increase in MWT and PWL and significant decrease in CXCR4, NLRP3, casepase-1, and IL-1β expression; compared with BCP+EA+Veh group, MWT and PWL were significantly decreased and CXCR4, NLRP3, casepase-1, IL-1β expression was significantly increased in BCP+EA+SDF-1 group. Conclusion: Electroacupuncture treatment can alleviate nociceptive hypersensitivity in BCP model mice, and this effect may be related to the inhibition of CXCR4 pathway activation by electroacupuncture.
