基于非靶向代谢组学策略探究银质针导热疗法干预肌筋膜疼痛综合征的机制*

Study on the mechanism of Silver Needle Thermotherapy in myofascial pain syndrome based on non-targeted metabolomics.*

摘要:

摘要 目的：采用非靶向代谢组学方法探讨银质针导热疗法治疗肌筋膜疼痛综合征作用机制。方法：采用局部钝性打击结合离心运动方式建立肌筋膜疼痛综合征（Myofascial pain syndrome, MPS）大鼠模型，于造模、治疗期间检测机械刺激缩足阈值（Mechanical withdrawal threshold，MWT），苏木精-伊红染色观察局部肌组织病理形态改变，超高效液相色谱与高分辨质谱联用技术对局部肌组织进行代谢图谱检测，检测与银质针导热疗法治疗相关的代谢变化，探索银质针导热疗法治疗MPS可能的机制。结果：与模型组相比，治疗组机械痛阈显著下降，病理形态改善明显。代谢组学鉴定发现其干预机制涉及的主要代谢通路包括胆碱代谢、甘油磷脂代谢、色氨酸代谢等。结论：银质针导热疗法治疗MPS机制涉及局部肌组织胆碱代谢、甘油磷脂代谢、色氨酸代谢。

Abstract:

Abstract Objective: To investigate the mechanism of silver needle therapy in the treatment of myofascial pain syndrome (MPS) rats by non-targeted metabolomics. Methods: Local blunt blow combined with eccentric exercise established myofascial pain syndrome(MPS) rat model, mechanical withdrawal threshold(MWT) was detected during modeling and treatment, the pathological morphological changes of local muscle tissue were observed by hematoxylin-eosin staining, and the metabolic profiling of local muscle tissue was detected by ultra-high performance liquid chromatography coupled with high-resolution mass spectrometry, and the metabolic changes related to the treatment of silver needle therapy were explored. To explore the possible mechanism of silver needle therapy in the treatment of MPS. Results: Compared with the model group, the MWT in the treatment group was significantly reduced, and the pathological morphology was significantly improved. Metabolomics identified the main metabolic pathways involved in intervention mechanisms included choline metabolism, glycerophospholipid metabolism, tryptophan metabolism and so on. Conclusion: The mechanism of silver needle therapy in the treatment of MPS involves local muscle tissue choline metabolism, glycerophospholipid metabolism, and tryptophan metabolism.