基于LC-MS/MS的慢性疼痛模型大鼠脊髓的蛋白质组学分析

Proteomic analysis of spinal cord in chronic pain model rats based on LC-MS / MS

摘要:

目的：采用蛋白质组学技术,观察正常组和模型组大鼠脊髓蛋白标志物,及各差异蛋白所参与的生物学过程,来探讨坐骨神经痛的发生机制。方法：将12只SD雄性大鼠随机分为正常组、模型组,每组6只。模型组在大鼠左侧坐骨神经进行结扎造模,通过定量蛋白质组学鉴定蛋白标志物并进行生物信息学分析。结果：从大鼠脊髓组织中共鉴定到5975个蛋白,其中正常组与模型组间有51个差异蛋白(DEPs),9个上调蛋白,42个下调蛋白。KEGG富集分析结果显示,差异蛋白主要涉及了丙酮酸代谢、辅助因子生物合成中的谷氨酸和谷氨酰胺代谢、胰岛素等信号通路。结论：坐骨神经痛的产生,其机制可能与丙酮酸代谢、胰岛素信号通路、谷氨酸和谷氨酰胺的代谢存在一定的关系。

Abstract:

Objective: Proteomics technology was used to observe the protein markers of spinal cord in normal group and model group, and the biological processes involved in different proteins, so as to explore the mechanism of sciatica.Methods: Twelve SD male rats were randomly divided into normal group and model group, six in each group. The model group was modeled by ligation of the left sciatic nerve in rats, and protein markers were identified by quantitative proteomics and analyzed by bioinformatics. Results: A total of 5975 proteins were identified from rat spinal cord tissues, including 51 differential proteins (DEPs), 9 up-regulated proteins, and 42 down-regulated proteins between the normal and model groups. KEGG enrichment analysis showed that the differential proteins were mainly involved in signaling pathways such as pyruvate metabolism, glutamate and glutamine metabolism in cofactor biosynthesis, and insulin.Conclusion: The mechanism of sciatica may be related to pyruvate metabolism, insulin signaling pathway, glutamate and glutamine metabolism.