天南星-生姜药对的抗炎镇痛活性研究

Study on Anti-inflammatory and Analgesic Effect of Rhizoma arisaematis-Zingiber Drug Pair

摘要:

目的 初步探究天南星-生姜药对的抗炎镇痛活性。方法 采用角叉菜胶致大鼠足跖肿胀实验和二甲苯致小鼠耳肿胀实验观察不同剂量天南星-生姜的抗炎效果；采用醋酸扭体实验和热板试验观察不同剂量天南星-生姜的镇痛作用。结果 在抗炎实验中，高剂量组天南星-生姜在前2小时可明显抑制大鼠足跖肿胀（P＜0.01），而中、低剂量组效果不显著；在二甲苯致小鼠耳肿胀实验中，高（P＜0.01）、中、低（P＜0.05）剂量组天南星-生姜均可可明显抑制小鼠耳肿胀，抑制效果呈剂量依赖性。在醋酸扭体实验中，高剂量组天南星-生姜可明显减少腹腔注射醋酸引起的小鼠扭体次数（P＜0.01），中、低剂量组也能起到良好的效果（P＜0.05）；在热板试验中，天南星-生姜对小鼠热痛阈值仅有轻微作用，无统计学意义。结论 天南星-生姜具有良好的抗炎作用，其外周镇痛作用较强，中枢镇痛效果不明显，且存在一定的剂量依赖性。

Abstract:

Abstract Objective: To investigate the anti-inflammatory and analgesic activity of Rhizoma arisaematis-Zingiber drug pair. Methods: The anti-inflammatory effect of different doses of Rhizoma arisaematis-Zingiber was observed by carrageenan-induced rat paws edema on SD rats and xylene-induced ear edema on ICR mice, while its analgesic activety was evaluated by acetic a cid-induced writhing test on ICR mice and hot-plate test. Results: In the anti-inflammatory test, the high-dose group of Rhizoma arisaematis-Zingiber could significantly inhibit the edema on rats’ paws caused by carrageenan in the first 2 hours (P＜0.01), while the effect of medium and low dose groups was not significant. In the experiment of xylene-induced ear edema on ICR mice, the high-dose group of Rhizoma arisaematis-Zingiber could significantly inhibit the ear edema of mice (P＜0.01), while the medium and low dose groups could also effectively inhibit the thickness of ear edema of mice (P＜0.05). In the writhing test, the high-dose group of Rhizoma arisaematis-Zingiber could significantly reduce the writhing times induced by intraperitoneal injection of acetic acid in mice (P < 0.01), and the medium and low-dose groups could also play a good effect (P < 0.05), while in the hot-plate test, Rhizoma arisaematis-Zingiber had only a slight effect on the pain threshold of hot-plate in mice, which was not statistically significant. Conclusion: Rhizoma arisaematis-Zingiber drug pair has a good anti-inflammatory effect, while its peripheral analgesic effect is strong but central analgesic effect is not obvious, and there is a certain dose-response relationship.