按压对大鼠肌筋膜激痛点软组织张力的影响及其作用机制研究

Effect and Mechanism of Pressing on Soft Tissue Tension of Rats Myofascial Trigger Points

摘要:

摘要：目的：本研究通过测定大鼠激痛点软组织张力、DHPRα1、RyR、P物质、胆碱酯酶（AChE）、游离Ca2+和降钙素基因相关肽（CGRP）的含量，探讨按压对大鼠激痛点的影响及其可能的机制。方法：SD大鼠48只，随机分为4组：空白组、模型组、利多卡因组和按压组。用软组织张力测定仪检测大鼠激痛点软组织张力；用Western Blot法检测DHPRα1、Ryanodine（RyR）和乙酰胆碱酯酶（AChE）；用免疫组化法检测P物质和降钙素基因相关肽。比色法检测游离Ca2+。结果：与空白组比较，模型组激痛点软组织张力升高，组织中DHPRα1、RyR和AChE含量降低，Ca2+、P物质和CGRP含量增加。与模型组相比，按压组、利多卡因组激痛点软组织张力均降低，组织中Ca2+、P物质和CGRP含量均降低，DHPRα1、RyR和AChE含量升高。结论：按压可以有效降低激痛点软组织张力，机制与促进DHPRα1与RyR表达，降低Ca2+浓度，减少P物质与CGRP表达，促进AChE表达有关。

Abstract:

[Abstract] Objective: In this study, the soft tissue tension, DHPRα1, ryanodine (RyR), substance P, Acetyl cholinesterase (AChE), Ca2+ and calcitonin gene-related peptide (CGRP) in myofascial trigger points of rats were measured to explore the effect of pressing and its possible mechanism. Method: Forty-eight SD rats were randomly divided into four groups: blank group, model group, lidocaine group and pressing group. The soft tissue tension was gauged by soft tissue tensiometer; The DHPRα1, RyR and AChE were detected by Western blot; The substance P and CGRP were tested by immunohistochemistry. The content of Ca2+ was measured by colorimetry with test kit. Result: Compared with the blank group, the soft tissue tension and the content of substance P and CGRP in model group were increased while the content of DHPRα1, RyR and AChE decreased. Compared with the model group, the soft tissue tension and the content of Ca2+, substance P and CGRP were decreased in the pressing group and lidocaine group while the content of DHPRα1, RyR and AChE increased. Conclusion: The pressing can effectively reduce the soft tissue tension of myofascial trigger points by promoting the expression of DHPRα1 and RyR , reducing the concentration of Ca2+, reducing the expression of substance P and CGRP, and promoting the expression of AChE. Key words: The pressing; myofascial trigger points; rat; soft tissue tension; DHPRα1; RyR; mechanism