交感神经和肌卫星细胞对肌筋膜激痛点修复的影响

Effects of sympathetic nerves and muscle satellite cells in the repair of myofascial trigger points

摘要:

目的 探讨交感神经和肌卫星细胞在肌筋膜触发点修复过程中发挥的作用。方法 取8周龄雄性SD大鼠30只，平均分为5组。采用化学阻断剂6-羟基多巴胺阻断大鼠交感神经，再建立肌筋膜触发点模型。后检测各组大鼠血浆中5-羟色胺（5-Hydroxytryptamine, 5-HT）、去甲肾上腺素（Noradrenaline, NE）和肿瘤坏死因-α（Tumor necrosis factor-α, TNF-α）浓度及肌肉中肌细胞生成素（Myogenin, MyoG）和成肌分化抗原（Myogenic Differentiation Antigen, MyoD）基因浓度。结果 1. 注射对照组大鼠血浆中NE浓度显著高于其他各组（P＜0.05）； 2. 试验组和神经阻断组大鼠血浆中5-HT浓度显著低于其余3组（P＜0.05）；3. 注射对照组触发点局部MyoD浓度显著高于试验组、打击对照组、神经阻断组（P＜0.05）；4.各组大鼠双侧的MyoD和MyoG浓度无显著性差异。结论 1.交感神经切除会影响大鼠整体炎症状态；2. 交感神经切除对触发点局部肌卫星细胞的增殖分化无影响; 3.无法证实交感神经可通过影响肌卫星细胞的增殖分化来影响大鼠炎症状态。

Abstract:

Objective To find the effects of sympathetic nerves and muscle satellite cells in the repair of myofascial trigger points. Methods 30 SD rats were randomly divided into 5 groups, rats in the experimental group were injected with 6-OHDA to block the sympathetic nerves in the first stage; in the second stage, myofascial trigger points model was accomplished by combating combined with centrifugal movement. Them, we tested the concentration of TNF-α, NE, 5-HT in rat plasma and MyoD and MyoG concentrations in the muscle of trigger points. Result 1. The concentration of 5-HT in the plasma of the rats in the experimental group and the nerve block group was significantly lower than that in the other three groups (P<0.05); 2. The concentration of MyoD in the trigger points of the control group was significantly higher than that in the B, D and E groups, MyoG concentration in the control group was significantly higher than that in group B and C; 3. There was no significant difference in the concentration of MyoD and MyoG between the muscle of trigger points and contralateral muscle in each group. Conclusion 1. Sympathetic nerves resection affects the inflammatory of the trigger points; 2. Sympathetic nerves has no effect on the proliferation and differentiation of satellite cells in trigger points; 3. It can not be concluded that sympathetic nerves can affect the inflammatory by affecting the proliferation and differentiation of muscle satellite cells.