Abstract:[Abstract] Objective This study aimed to evaluate the effectiveness of a novel real-time navigation system using Mixed Reality technology for oval foramen puncture for V3 trigeminal neuralgia treatment. Methods A total of 16 patients with V3 trigeminal neuralgia were treated with radiofrequency thermocoagulation. A total of 8 patients were treated under the guidance of the MR , while 8 patients underwent puncture via routine method (routine treatment group). The puncture times, and immediate postoperative pain were recorded. The degree of immediate postoperative pain was indicated by visual analogue scale (VAS), the postoperative complications were observed. Results The two groups showed significant decrease in VAS after the operation (P<0.05). The puncture and operation times of the MR group were significantly lower than those of the routine treatment group (P<0.05). The postoperative complications of the MR group were significantly lower than routine group (P<0.05). Conclusion Novel real-time navigation system using Mixed Reality technology may decrease the time of oval foramen puncture and reduce complication. Therefore, this technique possesses clinical promotional value