妊娠期慢性疼痛对子代生长发育的影响机制研究

RESEARCH ON THE IMPACT AND MECHANISM OF CHRONIC PAIN DURING PREGNANCY ON THE GROWTH AND DEVELOPMENT OF OFFSPRING

摘要:

目的: 研究妊娠期慢性疼痛对子代生长发育的影响及其分子机制。方法:选取40只同批成功受孕小鼠，采用随机数字表法分为四组（n = 10）：正常对照组、假手术对照组、慢性疼痛组、慢性疼痛+优裕胎教组。分别检测各组子鼠体重、身长、尾长等生长发育指标，采用酶联免疫吸附法分别在不同时间点检测各组母鼠和子鼠血清中去甲肾上腺素（NE）和皮质醇（Cortisol）水平并进行比较分析。结果:与假手术对照组相比，慢性疼痛组娩出的子鼠体重较轻、尾长较短，且该组母鼠在围产期各时间点、该组子鼠在出生日和出生后第三周的外周血中NE与Cortisol水平显著增高（P＜0.05）。与慢性疼痛组相比，慢性疼痛+优裕胎教组子鼠体重较重、尾长较长，且该组母鼠在孕12天至分娩日、该组子鼠在出生日的外周血中NE与Cortisol水平显著降低（P＜0.05）。结论：孕期慢性疼痛造成母体NE和Cortisol水平升高可能是导致子鼠生长发育较差的分子机制，优裕胎教可通过改变上述递质的分泌从而有效改善子鼠生长发育状况。

Abstract:

Objective：To study the impact and its molecular mechanism of chronic pain during pregnancy on the growth and development of offspring. Methods: Forty pregnant mice in the same batch were randomly divided into four groups (n = 10): normal, sham operation (sham), chronic pain (CP), CP+ enriched environmental intervention (EEI). The growth and development indexes of offspring mice, such as weight, body length and tail length, were measured in each group. The enzyme-linked immunosorbent assay was used to detect the levels of norepinephrine (NE) and Cortisol in the serum of both pregnant mice and the offspring in each group at different time points. Results: Compared with the sham group, the offspring of the CP group were lighter in weight and shorter in tail length, the levels of NE and Cortisol in the maternal blood of the CP group were significantly higher at different time points during the perinatal period (P < 0. 05), the levels of NE and Cortisol in the blood of the offspring increased significantly on the day of birth and the third week after birth (P < 0.05). Compared with the CP group, the weight of the offspring in the CP + EEI group were heavier and the tail length were longer, and the levels of NE and Cortisol in maternal blood from the 12th day of gestation to the day of delivery were significantly decreased, the levels of NE and Cortisol in offspring blood on the day of birth were significantly decreased too (P < 0 05). Conclusion: The increase of maternal NE and Cortisol levels caused by chronic pain during pregnancy may be the molecular mechanism leading to the poor growth and development of the offspring. EEI can effectively improve the growth and development of the offspring by changing the secretion of the transmitters.