臭氧水治疗膝骨关节炎的临床疗效及对关节液细胞因子的影响

Effect of Ozone Water on Cytokines in Joint Fluid of Patients with Knee Osteoarthritis and Clinical Analsis. Hei Guang, Li Jianfeng, Zhang Jun, Zhuang Zhigang, Dong Tieli, pain Department of the Second Affiliated Hospital of Zhengzhou University, Zhengzhou 450014, China.Corresponding Author: Dong Tieli,Email: [e-mail]

摘要:

【】目的：观察关节腔注射臭氧水对治疗膝骨关节炎患者关节液中VEGF、IL-6、IL- 1β和TNF-α的影响,并评价其临床疗效。方法：选择符合诊断标准的膝骨关节炎患者98例,随机分为对照组和试验组,每组49例。对照组关节腔内注射复方倍他米松注射液,试验组关节腔内注射浓度为20ug/ml臭氧水。2组每周各注射1次,共3周。所有患者治疗前和疗程结束1周后采用酶联免疫吸附试验(ELISA)检测关节液中VEGF、IL-6、IL- 1β和TNF-α含量,并采用Lysholm评分评价其膝关节功能,并在治疗后1月时进行临床疗效评价。结果：2组患者治疗前关节液中VEGF、IL-6、IL- 1β、TNF-α和膝关节Lysholm评分含量差异无统计学意义(P＞0.05)。2组患者治疗后关节液中VEGF、IL-6、IL- 1β和TNF-α含量均低于治疗前水平,治疗后膝关节Lysholm评分较治疗前均明显增加(P＜0.05),试验组与对照组关节液中VEGF、IL-6、IL- 1β、TNF-α含量和膝关节Lysholm评分组间差异无统计学意义(P＞0.05)。对照组和试验组总有效率分别为91.8%和87.8%(c_²=0.227,P>0.05)。结论：臭氧水关节腔注射治疗可明显减少膝骨关节炎患者关节液中VEGF、IL-6、IL-1β和TNF-α的含量,抑制关节腔内的炎症反应。

Abstract:

【】 Objective：To observe the level change of of VEGF, IL-6, IL-1β and TNF-α in synovial fluid of patients with knee osteoarthritis that given by injection of ozone water. Methods: 98 cases of knee osteoarthritis were collected and randomly divided into control group (49) and experimental group(49). The compound betamethasone and ozone water with a concentration of 20 ug/ml was respectively injected into articular cavity for control and experimental group, once a week, a total of 3 weeks. The levels of VEGF, IL-6, IL-1β and TNF-αin synovial fluid were measured by enzyme-linked immunosorbent assay (ELISA) before and one week after treatment. The Lysholm score of knees were recorded at two time point, the effective rate of both groups were compared one month after treatment. Results: There was no significant difference in the levels of VEGF, IL-6, IL-1β, TNF-α and Lysholm score of knee both groups before treatment (P>0.05). The levels of VEGF, IL-6, IL-1β and TNF-αin the synovial fluid of the two groups were lower than those before the treatment (P<0.05),The Lysholm score of both groups after treatment were increased than before. The levels of VEGF, IL-6, IL-1, TNF-αand Lysholm score were not significantly different (P>0.05). Conclusion: The levels of VEGF, IL-6, IL-1β and TNF-α can significantly increased in synovial fluid of patients with knee osteoarthritis by injection of ozone water that inhibit the inflammatory response in articular cavity.