硬膜外注射脉络宁复合液对脊髓内星形胶质细胞反应性增生的作用

EFFECTS OF GLIAL ACTIVATION IN THE SPINAL CORD BY EPIDURAL INJECTING MAILUONING

摘要:

目的:探讨神经根炎症大鼠脊髓中神经胶质细胞纤维酸性蛋白(GFAP)的表达,以及硬膜外注入脉络宁复合液对脊髓内星形胶质细胞反应性增生的作用。方法:Wistar大鼠,雄性,42只,随机分为7组,正常组(A)、假手术组(B)、神经根炎组(C)、脉络宁复合液治疗组(D)、激素治疗组(E)、单纯脉络宁治疗组(F)、单纯利多卡因治疗组(G)。D、E、F、G各组均按0.6ml/kg从硬膜外给药治疗,A、B、C组给于等量生理盐水,1次/4天,于18天后灌注处死取材,取炎症神经根相邻的脊髓段石蜡包埋,行GFAP免疫组化染色。结果:C组GFAP表达明显上调,与其它各组相比,有显著差异性(P<0.01);经过治疗后,各组GFAP的表达均有一定程度的下调,其中D、E两组与A组相比,无统计学上的差异(P>0.05)。结论:神经根的炎性损伤可以激活相应节段脊髓内的星形胶质细胞,使GFAP表达明显上调,硬膜外腔注射脉络宁复合液治疗后,GFAP的表达下调,说明脉络宁复合液对神经根炎大鼠脊髓中星形胶质细胞的反应性增生有抑制作用。

Abstract:

Objective:To detect the expression of GFAP in radiculitis rats,and to explore the inhibition of glial activation in spinal cord by epidural injection with Mailuoning.Methods:42 Wistar rats were divided into seven groups(n=6,each group) randomly:normal group(A),sham operation group(B),radiculitis group(C),Mailuoning compounding treated group(D),hormone treated group(E),Mailuoning treated group(F) and lidocaine treated group(G).Immunohistochemical technique was used to examine the expression of GFAP at day 18 after operation.Results:Group C had the highest level of GFAP expression among the seven groups.The expression of GFAP was decreased through corresponding epidural injection in group D,E,F and G,especially in group D and E.There were no significant differences among group A,D and E(P>0.05).Conclusions:Radiculitis injury can cause the glial activation and high expression of GFAP.Glial activation can be restrained through epidural injection of Mailuoning compounding.