Objective: To observe the clinical effects of 3D-CT guided oval foramen locations in radiofrequency thermocoagulation for trigeminal neuralgia. Methods: 3D-CT guided oval foramen locations was performed in 20 patients via dorsal position using Hartel' s technique, so that the direction and position could be defined for trigeminal neuralgia. Results: All needles located in oval foramen. Pain was alleviated immediately with no serious complication occurred in all of the patients. Conclusion: 3D-CT guided oval foramen locations can increase the successful rate of puncture, enhance the safety and reduce the incidence rate of complication, can be popularized in the future.