乙状结肠痛诱导大鼠远位触液神经元Fos蛋白的表达

THE SIGMOID PAIN STIMULATION INDUCED EXPRESSION OF FOS IN THE DISTAL CEREBROSPINAL FLUID CONTACTING NEURONS

摘要:

目的：探讨远位触液神经元在乙状结肠痛刺激信息调控中的作用。方法：采用CB-HRP／Fos双重标记技术，观察乙状结肠痛大鼠远位触液神经元中Fos样免疫反应蛋白（Fos）表达的变化。结果：乙状结肠痛刺激后，不仅疼痛行为学评分显著增加，而且远位触液神经元内Fos表达的数量显著增加，与空白对照组相比有显著性差异。结论：远位触液神经元可能参与了机体对乙状结肠痛刺激信息传递或调控。

Abstract:

Objective: To investigate the involvement of the distal cerebrospinal fluid contacting neurons(CSF-CNs) in modulation of sigmoid pain stimulation.Methods: The CB-HRP/ Fos double-labeling method was used to observe the expression of Fos in CB-HRP positive neurons following the sigmoid pain stimulation.Results: After the injection of formalin into the sigmoid wall,pain score increased remarkably,and companied by the increased expression of Fos in the CB-HRP labeled neurons.Conclusion: The distal CSF-CNs may be involved in the processing of sigmoid pain.