腰椎间盘内破裂模型的建立

AN EXPERIMENTAL MODEL OF LUMBAR INTERNAL DISC DISRUPTION

摘要:

目的：建立腰椎间盘内破裂模型并行组织学和椎间盘造影CT观察。方法：15只山羊，手术损伤L5,6前部全层纤维环，L6，7后部纤维环内层，术后3周和3、6个月观察椎间盘造影CT的变化，并进行组织学检查，评定纤维环损伤后的病理改变。结果：组织学和椎间盘造影CT显示L4，5和L5,6在实验期间未发现有后部纤维环内破裂，L6,7后部纤维环内层破裂处未愈合，并在3、6个月时破裂向纤维环外部不断延伸，但均未穿破纤维环外层。结论：经山羊腰椎间盘前方人为损伤后部纤维环内层，可以可靠、一致地制备椎间盘内破裂动物模型。

Abstract:

Objective: To establish the experimental model of lumbar internal disc disruption and to observe the alteration of histology and CT/discography.Methods: 15 goats were subjected to surgery.L_(5,6) intervertebral discs were full-thickness ventral stab of annulus fibrosus,L_(6,7) intervertebral discs were stab of posterior internal annulus fibrosus.CT/discography and the histological examination were taken at the 3 weeks,3 and 6 months after operation,in order to assess the degenerative and pathological change of intervertebral discs.Results: It was observed in histology and CT/discography that L_(4,5) and L_(5,6) intervertebral discs were not found the disruption of posterior internal annulus fibrosus.The disruption of L_(6,7) posterior internal annulus fibrosus unhealed,enlarged and spreaded to the exterior annulus at 3,6 months after operation.Conclusion: Stabbing the posterior internal annulus fibrosus through goat anterior lumbar discs results in a reliable uniforml animal model of internal disc disruption.