实验兔胃扩张疼痛模型的建立及其机制

THE ESTABLISHMENT AND MECHANISM OF PAIN MODEL INDUCED BY EXPERTIMENTAL GASTRIC DISTENSION IN RABBITS

摘要:

目的：用自制的胃扩张装置建立胃扩张疼痛模型，证实该模型的可靠性，并探讨其机制。方法：健康成年新西兰大白兔16只，随机均分为阻滞组（B）和非阻滞组（NB），在麻醉下B组用高强度聚焦超声阻滞腹腔神经节，NB组行假手术；7天后安置自制的胃扩张装置，14天后，清醒状态下采用容量梯度扩张胃，连续监测胃压力、心率、肌电图及胃容量等变化。结果：兔胃扩张至一定压力可引起拟痛行为反应，肌电图（EMG）电位募集相应地增加。兔胃扩张的压力和容积呈正相关；B组出现拟痛行为反应的容积和压力均高于NB组（P〈0．01）；在出现拟痛行为反应前相同的容积增加引起的压力增高值B组大于NB组（P〈0．01）。结论：本文建立的兔胃扩张疼痛模型是一种可靠的疼痛模型。

Abstract:

Objective: To establish a pain model of stomach distention by self-made equipment and prove it's reliability.Methods: 16 healthy adult rabbits were randomly divided into two groups: group block(B) and group no block(NB).In group B,the rabbits' celiac ganglion were blocked by high intensity focused ultrasound under anesthesia.In group NB,the rabbits received sham operation.7 days later,rabbits received gastric distension in conscious state.14 days later,under consciousness state,rabbits were distended the stomach gradually.The following parameters were continuously monitored and recorded through the experiment:(1) the pressure of stomach,(2) the HR and EMG,(3) the capacity of stomach.Results: The pseudoaffective responses were induced when the pressure rose to certain point.EMG collection increased with the pressure.The gastric pressure was positively correlated with capacity(P<0.01).The pressure and capacity which could induce pseudoaffective responses were significantly higher in group B than that in group NB(P<0.01).The increased pressure which produced by the same capacity were significantly higher in group B than that in group NB(P<0.01).Conclusion: This stomach distension model is reliable for pain measurement.