家兔坐骨神经内注射阿霉素对后肢感觉和运动功能的影响

EFFECTS OF INTRANEURAL INJECTION OF ADRICAMYCIN INTO SCIATIC NERVE TRUNK ON SENSORY AND MOTOR FUNCTION OF HINDLIMBS IN RABBITS

摘要:

目的：观察坐骨神经内注射阿霉素对家兔后肢感觉和运动功能的影响,评价其对背根神经节（DRG）的逆行性切除术作用.方法：家兔24只,右侧与坐股韧带相交处的坐骨神经内注射1%阿霉素0.2 ml,左侧注射生理盐水作为自身对照.于注射前及注射后1～8周测定双侧下肢痛阈、疼痛回缩反应、下肢运动功能以及坐骨神经运动传导功能.结果：注射阿霉素和生理盐水后1、4和8周双侧坐骨神经运动传导功能与注射前比较无显著性差异（P＞0.05）.生理盐水侧1～8周痛阈与注射前比较无显著性差异（P＞0.05）,疼痛回缩反应正常.阿霉素侧1和2周痛阈低于注射前（P＜0.05）,4和8周高于注射前（P＜0.001）;1和2周疼痛回缩反应正常,3～8周疼痛回缩反应逐渐减弱或消失（P＜0.01）.1和2周,家兔双侧后肢出现轻微跛行的阳性率无显著性差异（P＞0.05）,3～8周双侧后肢运动功能正常.结论：坐骨神经内注射小剂量阿霉素能够对相应DRG产生化学切除术效果,坐骨神经支配区域痛觉随阿霉素注射时间的延长逐渐减弱或消失,但下肢运动功能不受影响.

Abstract:

Objective: To observe the sensory and motor function in rabbit hindlimbs after adriamycin intraneural injection into sciatic nerves and evaluate the effects of retrograde adriamycin dorsal root ganglionectomy. Methods: The study involved 24 rabbits. After general anesthesia, 0.2 ml 1% adriamycin was injected into the right sciatic nerves intersecting ischiofemoral ligament, while isodose 0.9% saline was injected into the same site of the left as control. Pain threshold, withdrawal reflex to pain stimulus, motor function and motor conduction function of bilateral sciatic nerve were measured before and 1~8 weeks after injection. Results: The difference in motor conduction function of bilateral sciatic nerves between before and 1,4 and 8 weeks after injection was not significant (P>0.05). Pain threshold of the side injected with saline was maintained and rebound reflex to pain stimulus was normal in 1~8 weeks after injection. Pain threshold of the side injected with adriamycin was lower than that of pre-injection in 1~2 weeks (P<0.05) and higher in 3~8 weeks (P<0.001). Moreover, the withdrawal reflex to pain stimulus was normal in 1~2 weeks and weakened even disappeared in 3~8 weeks (P<0.01). In 1 and 2 weeks, the difference in positive rate of mild lameness in hindlimbs between both sides was not significant (P>0.05). In 3~8 weeks, motor function of bilateral hindlimbs was normal.Conclusion: Minidose adriamycin injected into sciatic nerve has the effect of chemo-ganglionectomy on corresponding dor- sal root ganglion. The pain perception in the area of sciatic innervation was weakened or disappeared over time while the motor function in hindlimbs was not affected.