蛋白激酶C对内脏炎症痛模型大鼠脊髓背角神经元电活动的影响

PKC MEDIATES FORMALIN-INDUCED NOCICEPTIVE RESPONSE IN RAT SPINAL CORD

摘要:

目的：本实验采用福尔马林诱导的急性内脏炎症痛模型，观察蛋白激酶C（Protein Kinase C，PKC）激动剂PMA（phorbol 12-myristate 13-acetate）与抑制剂H-7（1-（5-isoquinolinesulfonyl）2-meth-ylpiperazine dihydrochloride）对脊髓背角神经元放电的影响。方法：选用健康成年Wistar大鼠，随机分为4组：（1）福尔马林直肠粘膜下注射致炎组（F）；（2）福尔马林致炎与脊髓微透析生理盐水组（F＋NS）；（3）福尔马林致炎与脊髓微透析H-7组（F＋H-7）；（4）福尔马林致炎与脊髓微透析PMA组（F＋PMA）。结果：记录到24个单位的反应结果表明：福尔马林致炎后0～120min内放电频率明显增加，特别是0～15min和15～30min内与致炎前相比均有显著性增强（P＜0．05）；经微透析PMA和H-7后，上述时间段内放电频率分别明显增强（P＜0．01）和回降（P＜0．05）。结论：PKC在福尔马林致内脏炎症痛的产生和维持中起重要作用。

Abstract:

Objective: In the present study,the effect of PKC activator and inhibitor on formatin evoked responses of spinal cord dorsal horn neurons were investigated.Methods: Healthy adult Wistar rats were randomly divided into four groups:(1) injected with formalin in rectum [F];(2) injected with formalin and treated with normal sailine through microdialysis in spinal dorsal horn ;(3) injected with formalin and treated with H-7 ;(4) injected with formalin and treated with PMA .Results: A total of 24 units were recorded.The discharge frequency of spinal neurons were increased significantly in 0~120min after formalin injection,especially in the former 30min(P<0.05).There were significant increase and decrease in groups of F+PMA(P<0.01) and F+H-7(P<0.05) respectively during the earlier 30min after formalin injection.Conclusion: The results suggest that PKC may play an important role in the development of visceral pain induced by formalin.