乙状结肠痛对大鼠中缝背核Fos-LI和NADPH-d阳性神经元表达影响的实验研究

INVOLVEMENT OF FOS-LI AND NADPH-d POSITIVE NEURONS IN RAT DORSAL RAPHE NUCLEUS IN THE SIGMOID PAIN

摘要:

目的：探讨中缝背核还原型尼克酰胺腺嘌呤二核苷酸脱氢酶(NADPH-d)阳性神经元是否参与调控大鼠乙状结肠痛。方法：采用Fos免疫组织化学、NADPH-d组织化学及Fos／NADPH-α双标技术，观察乙状结肠痛大鼠中缝背核NADPH—d阳性神经元和Fos样免疫反应蛋白(Fos-LI)的变化。结果：乙状结肠痛刺激后，中缝背核内NADPH-d阳性神经元个数和染色深度增加；中缝背核Fos—LI明显增加；中缝背核内有Fos-LI／NADPH-d双标神经元的表达，约占NADPH-d神经元的11％，与生理盐水对照组相比有显著性差异。结论：中缝背核NADPH-d阳性神经元可能参与对乙状结肠痛刺激的信息传递。

Abstract:

Objective: To investigate if the NADPH d positive neurons in dorsal raphe nucleus (DR) were involved in modulation of sigmoid pain. Methods: The Fos immunocytochemistry, NADPH d histochemistry and Fos LI /NADPH d double labeling methods were used to study the changes in NADPH d positive neurons and Fos LI in dorsal raphe nucleus (DR) of rats after formalin stimulation. Results: After the injection of formalin into the sigmoid wall, the expression of NADPH d positive neurons and the staining degree in the DR were increased remarkably; the expression of Fos like immunoreactivity (Fos LI) in DR was increased significantly; and about 11% Fos LI /NADPH d double labeled neurons were detected in NADPH d positive neurons in the DR. Conclusion: NADPH d positive neurons may be involved in the processing of pain induced by injecting formalin into the sigmoid wall in rats.