伤害性刺激、电针镇痛后大鼠中脑导水管周围灰质生长抑素mRNA的表达

EXPRESSION OF SOMATOSTATIN mRNA FOLLOWING NOXIOUS STMULATION AND ELECTROACUPUNCTURE ANALGESIA IN PERIAQUEDUCTAL GRAY MATTER

摘要:

目的：研究生长抑素（ＳＯＭ）镇痛效果的作用机制。方法：用原位杂交结合计算机图像分析，观察正常大鼠中脑导水管周围灰质（ＰＡＧ）内ＳＯＭ神经元的基因表达；伤害性刺激、单纯针刺和电针镇痛（ＥＡ）对ＰＡＧ内ＳＯＭ多肽合成的调控，以探讨伤害性刺激和电针对ＰＡＧ内ＳＯＭ在基因转录水平的激活影响。结果：正常情况下ＰＡＧ内有一定的ＳＯＭｍＲＮＡ表达，伤害性刺激和单纯电针后ＳＯＭｍＲＮＡ表达增强，ＥＡ后进一步增强。结论：（１）ＳＯＭ可能参与痛觉调制；（２）ＳＯＭ可能参与ＥＡ。

Abstract:

Aim: To study the possible role of somatostatin in analgesia. Methods: In situ hybridization (ISHH) and image processing were used to study the change of somatostatin (SOM) mRNA level in periaqueductal gray matter (PAG) of rats in the normal group, noxious stimulation group, simple acupuncture group and electroacupuncture analgesia group (EA). Results: In normal group, SOM mRNA positive cells with intense hybridization signal were mainly oval and concentrated principally in ventromedial, ventrolateral subnuclei of PAG. An increase of SOM mRNA positive cells was seen in noxious stimulation group and simple acupuncture group comparing with the normal group. In EA group the number of SOM mRNA positive cells increased more than any other groups. Conclusion: 1. SOM may take part in pain modulation. 2. SOM may play a role in EA.